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CPC
Purification Process
Centrifugal Partition Chromatography (CPC) is essentially a liquid-liquid partitioning technique that eliminates various complications arising from the use of solid supports. Separation takes places in the centrifuge using two immiscible liquid phases consisting typically of a mixture of 2 to 4 solvents in a specific ratio. During a chromatographic run one phase (stationary) is immobilized by centrifugal force while the other (mobile phase) is pumped through at relatively high flow rates. Fractionation of compounds is then accomplished by partitioning of sample components between the two phases.
The features and benefits of the emerging Centrifugal Partition
Chromatography (CPC) primarily cover the technological and economical
advantages:
- replacing and improving the majority of prep HPLC applications;
- higher capacity and superior resolution by optimal solvent
selection combinations;
- cost reduction by cheaper (recyclable) solvents versus
expensive solid support material;
- no loss of expensive and delicate target compounds, excellent
sample recovery, no absorption on solid phase;
- highly suitable for fragile biotech sample extractions
and purifications;
- normal phase and reverse phase separation in-one-go.
The wide choice of CPC solvent systems means that the whole spectrum of polarities can be covered and compounds ranging from very hydrophilic to extremely lipophilic can be fractionated. Using solvents varying from water to acetone to hexane, a suitable solvent system can be developed for every demand. Methods can be easily transferred to another apparatus, making the scaling up resulting in CPC large scale separations and isolations at relatively low costs.
Some examples where we have applied the CPC successfully for the separation and isolation of compounds of interest are:
- Beer brewing process: Bitter compounds from the
Hops plant;
- Medicinal cannabis: Cannabinoids from the cannabis
plant for clinical testing;
- Cosmetic research: Coloring agents and skin products
from selected plants;
- Medical research: Acetylcholine esterase (AchE)
inhibitors from Narcissus and Tulip bulbs;
- Traditional medicine: Anti-asthmatic compounds
from Thai medicinal plants
- Cancer research: Alkaloids with anti-cancer activities
from Catharanthus roseus cell-culture extracts.
Some of these applications are recently under development for large scale production for the market. CPC production under a GMP regime is done for compounds that are targeted for the clinical market |
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