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NMR
analysis
For Quality Control and Chemical Analysis purposes, PRISNA offers
NMR-services being:
- full structure elucidation of unknown compounds or impurities;
- confirmation of identity of active principles;
- quantification when no reference standards are available;
- fingerprinting as part of quality control;
- experience with principle components analysis (PCA);
- interaction studies (complexation, ligand binding);
- spectrum interpretation.
Hydrogen-1 (1H) and carbon-13 (13C) NMR spectroscopy for chemicals
are indispensable to the organic chemist in identifying the
structures of virtually all organic compounds, no matter how
complex. It is even being used to determine very complex structures
such as enzymes and proteins.
The analysis is quick and simple and does not require an especially
pure sample. NMR produces a spectrum containing a number of
peaks. The heights and positions of these peaks enables a chemist
to very accurately determine what the carbon-hydrogen framework
of an organic molecule is. Measurements with NMR are non-destructive,
which means that delicate and valuable samples are returned
essentially unchanged.
Applications NMR:
- to measure the water/fat ratio in foods,
- to monitor the complexation of sample components
- to determine the absolute purity of active pharmaceutical
ingredients (API)
The application of NMR screening in drug discovery has recently
attained heightened importance throughout the pharmaceutical
industry. Structural data is critical in determining how a potential
drug will bind with a target molecule. As part of structure-activity
relationship (SAR) studies, nuclear magnetic resonance screening
of multiple targets and the rational design of high-affinity
ligands are now a main focus of drug discovery efforts. NMR
screening can be applied at various points in a drug discovery
program, ranging from very early in the program, when new targets
can be screened long before an HTS enzymatic assay is developed,
to later in the program, as in the case where no useful hits
have been detected by HTS using biological assays. |
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